biofilm formation and β-lactamase production in burn isolates of pseudomonas aeruginosa
نویسندگان
چکیده
conclusions biofilm formation (both strong and weak) strongly correlated with psla gene carriage. biofilm formation also correlated with mbl and ampc β-lactamase production. more importantly, multiple-β-lactamase phenotype was associated with formation of strong biofilms. results biofilm formation was observed in 43.5% of the isolates, of which 66.7% produced strong and 33.3% formed weak biofilms. all biofilm-positive and 14.2% of biofilm-negative isolates harbored the psla gene. mbl, ampc and esbl production were significantly higher in the biofilm-positive isolates (70.3%, 62.9% and 33.3%, respectively) compared to the biofilm-negative strains (31.4%, 34.2% and 20%, respectively). overall, 19 isolates (30.6%) co-produced mbl and ampc, among which the majority were biofilm-positive (63.1%). finally, four isolates (6.4%) had all three enzymes, of which 3 (75%) produced biofilm. objectives the aim of this study was to evaluate biofilm formation and presence of the psla gene in burn isolates of p. aeruginosa as well as the association of antibiotic resistance, mbl, esbl and ampc β-lactamase production with biofilm formation among the isolates. materials and methods sixty-two burn isolates of p. aeruginosa were obtained from shahid motahari hospital in tehran from august to october 2011. antibiotic susceptibility was determined by the disc diffusion assay. mbl, ampc and esbl production were screened using the double disc synergy test, ampc disc test and combined disc diffusion assay, respectively. the potential to form biofilm was measured using the microtiter plate assay and psla gene was detected using specific primers and pcr. background pseudomonas aeruginosa is an important nosocomial pathogen characterized by its innate resistance to multiple antimicrobial agents. plasmid-mediated drug resistance also occurs by the production of extended-spectrum β-lactamases (esbl), metallo β-lactamases (mbl), and ampc β-lactamases. another important factor for establishment of chronic infections by p. aeruginosa is biofilm formation mediated by the psl gene cluster.
منابع مشابه
Biofilm Formation and β-Lactamase Production in Burn Isolates of Pseudomonas aeruginosa
BACKGROUND Pseudomonas aeruginosa is an important nosocomial pathogen characterized by its innate resistance to multiple antimicrobial agents. Plasmid-mediated drug resistance also occurs by the production of extended-spectrum β-lactamases (ESBL), metallo β-lactamases (MBL), and AmpC β-lactamases. Another important factor for establishment of chronic infections by P. aeruginosa is biofilm forma...
متن کاملPrevalence of Extended-Spectrum and Metallo β-Lactamase Production in AmpC β-Lactamase Producing Pseudomonas aeruginosa Isolates From Burns
BACKGROUND Pseudomonas aeruginosa is one of the most common causes of nosocomial infections. Resistance of P. aeruginosa to β-lactam antibiotics may be the result of acquired resistance through mutation and over production of various antibiotic inactivating enzymes. This research aimed to determine the prevalence of extended-spectrum β-lactamases (ESBL) and metallo β-lactamase (MBL) production ...
متن کاملprevalence of extended-spectrum and metallo β-lactamase production in ampc β-lactamase producing pseudomonas aeruginosa isolates from burns
conclusions a high prevalence of multiple β-lactamase production was observed among the ampc producers (60%), of which the majority co-produced ampc and mbl. the current study results showed correlation between β-lactamase production and the presence of antibiotic resistance genes in the isolates. results all isolates were multidrug resistant. ampc, esbl and mbl production were observed in 35 (...
متن کاملcoexistence of ampc and extended-spectrum β-lactamases in metallo-β-lactamase producing pseudomonas aeruginosa burn isolates in tehran
conclusions this study showed that multiple β-lactamases can be produced in burn isolates. this suggests that use of cephalosporins and carbapenems should be restricted in burn isolates to minimize the development and spread of these multidrug resistant pathogens. results there was 99% resistance to carbenicillin, and ticarcillin, 98% to cotrimoxazole, 96% to ciprofloxacin, and aztreonam, 95% t...
متن کاملMetallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread.
The aim of this study was to characterize two metallo-β-lactamases (MBLs)-producing Pseudomonas aeruginosa clinical isolates showing meropenem susceptibility. Antimicrobial susceptibility was assessed by automated testing and Clinical and Laboratory Standards Institute agar dilution method. MBL production was investigated by phenotypic tests. Molecular typing was determined by pulsed field gel ...
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عنوان ژورنال:
jundishapur journal of microbiologyجلد ۸، شماره ۳، صفحات ۰-۰
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